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The residue was re-dissolved in 1 mL methanol, filtered through 0.2 mM filters for RP-HPLC analysis by a method by G orna s et al. [7]. Chromatographic separation of the extract (20 mL) was
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MF-Millipore™ Membrane Filter, 0.45 µm pore size 47 mm diameter, mixed cellulose esters (MCE) membrane, hydrophilic, white, 100 discs - Find MSDS or SDS, a COA, data sheets and more information.
Samples were dissolved at a concentration of 0.5 mg/mL in sample solvent of 0.1 M sodium phosphate at pH 7.4 with 0.025% sodium azide, heated for 1 min at 65 C, and filtered with a 0.2-lm syringe
CHM® SNY syringe filters offers a nylon merane in a polypropylene housing. Due to their high chemical compatibility and physical strength, these syringe filters are recommended for clarifying and sterilizing HPLC samples up to 200 ml volume. They are supplied in two pore sizes, 0.2 and 0.45 µm, and in two diameters 13 and 25 mm.
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