7/5/2021 · How to sterilize MCE membrane filter. 1. Steam sterilization for 30 minutes. 2. Autoclave sterilization (121℃, 30min). Soak the filter membrane in distilled water for over half an hour, keep it moist during the sterilization process. Disinfect the filter membranes of all appliances if the not good wetting affects the flow rate.